Influenza-specific antibody-dependent phagocytosis

F Ana-Sosa-Batiz; H Vanderven; S Jegaskanda; A Johnston; S Rockman; K Laurie; I Barr; P Reading; M Lichtfuss; SJ Kent

Journal article

Influenza-specific antibody-dependent phagocytosis

F Ana-Sosa-Batiz, H Vanderven, S Jegaskanda, A Johnston, S Rockman, K Laurie, I Barr, P Reading, M Lichtfuss, SJ Kent

Plos One | PUBLIC LIBRARY SCIENCE | Published : 2016

DOI: 10.1371/journal.pone.0154461

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Abstract

Background: Immunity to human influenza A virus (IAV) infection is only partially understood. Broadly non-neutralizing antibodies may assist in reducing disease but have not been well characterized. Methods: We measured internalization of opsonized, influenza protein-coated fluorescent beads and live IAV into a monocytic cell line to study antibody-dependent phagocytosis (ADP) against multiple influenza hemagglutinin (HA) subtypes. We analyzed influenza HA-specific ADP in healthy human donors, in preparations of intravenous immunoglobulin (IVIG), and following IAV infection of humans and macaques. Results: We found that both sera from healthy adults and IVIG preparations had broad ADP to mul..

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University of Melbourne Researchers

Steven Rockman Author

Ian Barr Author

Patrick Reading Author

Stephen Kent Author

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Awarded by Seventh Framework Programme

Funding Acknowledgements

This work was supported by the Australian National Health and Medical Research Council [SJK 628331, 1023294, and 510488; https://www.nhmrc.gov.au/]. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health [http://www.health.gov.au/]. The funder provided support in the form of salaries for authors SJK, SJ, KL, IB, and PR, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section.